Software for automated data evaluation
In order to facilitate rapid screening of compounds affecting kinesin motor activity as well as optimizing kinesin-based in vitro motility assays for nanotechnological applications, we developed an in vitro motility assay where sample preparation, imaging and data evaluation are fully automated, enabling the processing of a 384-well plate within less than three hours. We demonstrate the automated assay for the analysis of peptide inhibitors for kinesin-1 at a wide range of concentrations.
The source code for the automated data evaluation is available on GitHub: https://github.com/thawn/AutoTipTrack
Korten, T., Tavkin, E., Scharrel, L., Kushwaha, V. S. & Diez, S. An automated in vitro motility assay for high-throughput studies of molecular motors. Lab on a Chip 18, 3196-3206, doi:10.1039/c8lc00547h (2018).